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Sepharose® 6B

Cytiva 17-0110-01, pack of 1 L

Agarose, Beaded
CAS Number:
MDL number:

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


autoclavable Autoclavable, 20 min at 120°C in pH 7


pack of 1 L


Cytiva 17-0110-01


6% agarose

particle size

45-165 μm

cleaning in place


working range


SMILES string




InChI key


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General description

Sepharose® 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Sepharose® is a bead-formed agarose-based gel filtration matrix. Sepharose® is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose® 2B, Sepharose® 4B and Sepharose® 6B respectively, Both Sepharose® and Sepharose® CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.

Sepharose® CL gels are cross-linked derivatives of Sepharose® 2B, Sepharose® 4B and Sepharose® 6B. The cross-linked form of Sepharose® is chemically and physically more resistant than Sepharose® itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose® gels are resistant to organic solvents and are thus the choice for separations in organic solvents.

Features and Benefits

  • 6% Agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands

Other Notes

Beaded agarose for fractionating molecules of high molecular weight. Cross-linked beaded agarose is more resistant to denaturing conditions, and thus offers more versatility in the choice of sample buffer and eluent. The approximate % agarose concentration is indicated by the product name (Ultrogel A%).

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

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Legal Information

Sepharose is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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More Documents

Quotes and Ordering

Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
N C Stellwagen et al.
Electrophoresis, 14(4), 355-368 (1993-04-01)
The orientation of the agarose gel matrix in pulsed electric fields has been studied by transient electric birefringence. Two types of agarose with different degrees of charge were studied, in addition to agarose solutions and gels containing beta-carrageenan, a stereoisomer
P Upcroft et al.
Journal of chromatography, 618(1-2), 79-93 (1993-08-25)
Agarose as a medium for separation of DNA was first introduced in 1962 and since the early 1970s agarose submarine gel electrophoresis has been synonymous with separations of DNA molecules larger than 1 kilobase pair (kb). The large pore size
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized

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