CG201

cGMP Enzyme Immunoassay Kit

sufficient for 96 assays

• UNSPSC Code: 12161503
• NACRES: NA.32

Properties

• usage: sufficient for 96 assays
• Quality Level: 200
• technique(s): ELISA: suitable
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PRKG1(5592)

Description

General description

cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The immunoassay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

Application

Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues and mouse aortic artery homogenates. It has also been used to assess the guanylate cyclase activity.

Kit Components Only

• Acetic Anhydride 1 x 1
• Assay Buffer 2 1 x 30
• cGMP-Alkaline Phosphatase Conjugate 1 x 5
• cGMP Assay Layout Sheet 1 x 1
• cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
• 5 Cycle Logit-Log Paper 1 x 1
• Cyclic GMP Standard 1 x 0.5
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
p-Nitrophenyl Phosphate Substrate Solution 1 x 20
Plate Sealer 1 ea
Stop Solution 1 x 5
Triethylamine 1 x 2
Wash Buffer Concentrate 1 x 30

See All (13)

Safety Information

• pictograms: GHS02,GHS06,GHS05
• signalword: Danger
• hcodes: H225,H302,H311,H314,H330,H335
• pcodes: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 12.2 °F - closed cup
• flash_point_c: -11 °C - closed cup