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AtT-20/D16v-F2

94050406

biological source

mouse brain

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse pituitary tumour

Cell Line Description

This F2 subclone was developed from original AtT-20, an andrenocorticotropic hormone (ACTH) secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.

Application

AtT-20/D16v-F2 has been used to study the role of the G-protein Rasd1 in hypothalamic response to neuronal activation. It has also been used to study the effect of cAMP and glucocorticoid treatment on cAMP-responsive element binding protein 3 like 1 (CREB3L1).

Culture Medium

DMEM + 2mM Glutamine + 10% FBS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cell clumps must be broken up to prevent cells growing in suspension.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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