EoL-1 cell

94042252

• Synonym(s): AML-EOL-1 Cells, EoL1 Cells, Eosinophilic Leukemia Cell Line
• UNSPSC Code: 41106514

Properties

• biological source: human blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human eosinophilic leukaemia

Cell Line Description

Differentiated by N-butylate treatment.

Application

EoL-1 cell line has been used to study the role of AGS67E, an antibody-drug conjugate that targets cluster of differentiation-37 (CD37) for the treatment of B/T-cell malignancies. It has also been used to study the role of anti-vascular cell adhesion molecule-1 (VCAM-1) monoclonal antibody in inhibiting asthma in a murine asthma model.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 12,13
D16S539: 8,10
D5S818: 11
D7S820: 10,13
THO1: 9.3
TPOX: 8
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Eol-1 cells grow very slowly and may take up to 5 days to reach a suitable level to subculture. It is best that these cells are left to grow and not agitated more than necessary.The cells actually grow as large clusters. They are beginning to reach confluence when the cells in the centre of each cluster start to look unhealthy. The change in media colour should also be an indication. Eol-1 cells prefer slightly acidic conditions and so may resuscitate better from frozen if started up in 15% foetal bovine serum.

Other Notes

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