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SCC067

Sigma-Aldrich

HMC-1.1 Human Mast Cell Line

HMC-1.1 human mast cell line is a variant subline of the HMC-1 cell line and possesses the G560V KIT mutation.

Synonym(s):
HMC-1 cell line
eCl@ss:
32011203

Quality Level

biological source

human

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

Related Categories

General description

HMC-1 cell line was established from a patient with mast cell leukemia. HMC-1 cells are widely used in studies of human mast cell function because they exhibit many key characteristics of tissue mast cells, such as expression of histamine, tryptase, heparin and similar cell surface antigen-profile (1,2). Receptor tyrosine kinase KIT is expressed on mast cells and plays an important role in the proliferation, function and survival of mast cells. Activating mutations in KIT have been linked to dysregulated growth of mast cells and are associated with mast cell tumors and systemic mastocytosis. Two activating point mutations in KIT have been reported in the HMC-1 cell line (3). Both mutations convert the receptor to a constitutively tyrosine phosphorylated and active state, leading to growth factor-independent proliferation.

1. Nilsson, G, et al. (1994) Phenotypic Characterization of the Human Mast-Cell Line HMC-1. Scand. J. Immunol 39: 489-498.
2. Sundstrom, M, et al. (2003) Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c-kit proto-oncogene. Immunology 108: 89-97.
3. Furitsu, T, et al. (1993) Identification of mutations in the coding sequence of the proto-oncogene c-kit in a human mast cell leukemia cell line causing ligand-independent activation of c-kit product. J. Clin. Invest. 92(4): 1736-4.
HMC-1.1 (HMC-1(560)) and HMC-1.2 (HMC-1(560,816)) are two variant sublines of the HMC-1 cell line (2). HMC-1.1 possess the G560V mutation, but not the D816V mutation. HMC-1.2 possess both the G560V and D816V mutations and exhibits a higher proliferative rate than HMC-1.1. It has been suggested that the higher proliferative potential of HMC-1.2 may be attributed to the D816V mutation.

Cell Line Description

Cancer Cells

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Research Category
Inflammation & Immunology

Cancer

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

Certificate of Origin

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