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Key Documents

F10-89-4

89062101, Reactivity: Anti-CD45 determinant 1, human

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS1/1

Reactivity

Anti-CD45 determinant 1, human

technique(s)

cell culture | mammalian: suitable

isotype

IgG2a

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse NS1/1 x Mouse BALB/c spleen

Cell Line Description

The antibody recognises the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83% bone marrow cells and granulocytes. The antigen recognised by F10-89-4 is a glycoprotein with a molecular weight of 190kDa to 215kDa.

Immunogen

Human T lymphocytes

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder cells upon resuscitation

Other Notes

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