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Jurkat E6.1 Cell Line human

88042803, from human blood(leukemic T-cell lymphoblast)

biological source

human blood (leukemic T-cell lymphoblast)

growth mode



Pseudodiploid, modal No 46


Not specified


Interleukin 2 (IL-2), human alpha interferon


Not specified


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Human leukaemic T cell lymphoblast

Cell Line Description

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.


Jurkat E6.1 cell line has been used to:
  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro
IL-2 assay and cloning studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Other Notes

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Certificate of Analysis

Certificate of Origin

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Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

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