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A7r5 Cell Line from rat

86050803

biological source

rat muscle

description

Rat BDIX smooth muscle embryonic aorta

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Muscle-type isoenzymes

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat BDIX smooth muscle embryonic aorta

Cell Line Description

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised.

Application

Biochemical and biophysical studies
A7r5 Cell Line from rat has been used to study the effect of angiotensin II on vascular oxidative stress. It has been used in PLA2 inhibitory study to understand the role of PLA2 on formation of lipid droplets.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

Other Notes

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Certificate of Analysis

Certificate of Origin

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