product name
P815-1-1, 85011406
biological source
mouse breast
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse DBA/2 mastocytoma
Cell Line Description
From DBA/2 mouse tumour treated with methylcholanthrene. Used as target cell for cytotoxic T-cell assays. Cells exhibit no effector activity in antibody dependent cell mediated cytotoxic system. Growth not inhibited by dextran sulphate, LPS or PPD.
Application
Cytotoxicity and tumourigenicity studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml. The cells are non-adherent but can give rise to adherent cells with large granules; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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