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C6-2-3

08062528

biological source

rat brain

growth mode

Adherent

karyotype

modal No varied with passage see reference

morphology

fibroblastic

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat glioma x rat glioma hybrid

Cell Line Description

A pseudotetraploid call line generated by fusion of 8-bromodeoxyuridine-resistant mutant rat glioma cell line C6-BU-1 and the 6-thioguanine-resistant mutant rat glioma cell line C6-TG-1.

Application

electrophysiology

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C

Other Notes

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Certificate of Analysis

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Certificate of Origin

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