Cell Line Origin
Human colon adenocarcinoma
Cell Line Description
Established from an adenocarcinoma located in the ascending colon of a 68 year-old male patient. The adenocarcinoma was well differentiated and determined to be Dukes′ stage B. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split subconfluent cultures (70-80%) 1:2 to 1:10 using 0.25% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm2.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.