HCA-24

06061903

Properties

• biological source: human colon
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human colon adenocarcinoma

Cell Line Description

Established from an adenocarcinoma located in the ascending colon of a 68 year-old male patient. The adenocarcinoma was well differentiated and determined to be Dukes′ stage B. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:10 using 0.25% trypsin/EDTA; 7.5% CO₂; 37°C. Suggested seeding density 2-4x10,000 cells/cm².

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.