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C7559

Sigma-Aldrich

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
MDL number:
PubChem Substance ID:

grade

PCR Reagent

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

form

liquid

contains

amylenes as stabilizer

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, none detected

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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General description

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent.[33] Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.[58]

Application

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids.[86] It has also been used in RNA extraction/isolation[58] and purification.[66]

Packaging

5 vials in serum bottle

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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