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Key Documents

C7401

Sigma-Aldrich

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Anti-Con A, Anti-Jack Bean Concanavalin A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

Canavalia ensiformis

packaging

vial of ~2 mL lyophilized antiserum

technique(s)

indirect ELISA: 1:25,000

storage temp.

2-8°C

target post-translational modification

unmodified

Immunogen

Jack Bean Concanavalin A (Con A).

Application

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assays (ELISA)
  • immunochemistry
  • enhanced chemiluminescence ECL western blot analysis
  • western blot

Biochem/physiol Actions

Concanavalin A is a lectin isolated from Canavalia ensiformis or Jack bean seeds. It has potent mitogenic effect on T cells and triggers the activity of both helper and suppressor T cells exhibiting immune regulator activity. Con A triggers autophagy by binding on the cell membrane glycoprotein and is internalized to the mitochondria. It activates immune response in liver and displays anti-hepatoma effect.

Target description

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Reconstitution

Reconstitute with 2 mL deionized water.

Storage and Stability

Prior to reconstitution store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.

Legal Information

ECL is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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