C4859

Cycloheximide solution

Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture

• Synonym(s): CHX
• Empirical Formula (Hill Notation): C₁₅H₂₃NO₄
• CAS Number: 66-81-9
• Molecular Weight: 281.35
• MDL number: MFCD00082346
• PubChem Substance ID: 24892732
• NACRES: NA.77

Properties

• biological source: microbial
• Quality Level: 300
• description: suitable for testing specifications (CHO C. growth inhibitor)
• form: solution
• concentration: 100 mg/mL in DMSO
• solubility: DMSO: soluble
• antibiotic activity spectrum: fungi; yeast
• Mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C
• InChI: 1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
• InChI key: YPHMISFOHDHNIV-FSZOTQKASA-N
• Gene Information: human ... FKBP1A(2280) , PIN1(5300)

Description

Specificity

Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Hygroscopic. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301 - H315 - H319 - H341 - H360D - H412
• Precautionary Statements: P202 - P264 - P273 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup