C3640

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C3640

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

All Photos(1)

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II

CAS Number:

9001-03-0

Enzyme Commission number:

4.2.1.1 (BRENDA, IUBMB)

EC Number:

232-576-6

MDL number:

MFCD00081467

NACRES:

NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥2,000 W-A units/mg protein

pI

~5.4

storage temp.

−20°C

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1 of 4

This Item	C7025	C3934	C2522
Sigma-Aldrich C3640 Carbonic Anhydrase Isozyme II from bovine erythrocytes	Sigma-Aldrich C7025 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C3934 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C2522 Carbonic Anhydrase Isozyme II from bovine erythrocytes
form lyophilized powder	form powder	form lyophilized powder	form lyophilized powder
specific activity ≥2,000 W-A units/mg protein	specific activity -	specific activity ≥2,000 W-A units/mg protein	specific activity ≥3,000 W-A units/mg protein
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300
pI ~5.4	pI -	pI -	pI ~5.9

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme. The enzyme has also been used to generate CD₄⁺ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in pathologies such as glaucoma, epilepsy and Parkinson′s disease.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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