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C-28030

Sigma-Aldrich

Mononuclear Cell Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

Mononuclear Cell Medium (Ready-to-use)

Application

Mononuclear Cell Medium is a very rich short-term maintenance medium, which provides utmost cell viability for Mononuclear Cells from umbilical cord blood, adult peripheral blood and other sources, e.g. bone marrow. It is suitable to maintain Mononuclear Cells for up to 48 hours before proceeding with your experiments.Recommended for:Human Mononuclear Cells from Cord Blood (hMNC-CB)Human Mononuclear Cells from Peripheral Blood (hMNC-PB)

Quality

All lots of PromoCell Mononuclear Cell Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from cord blood and adult peripheral blood. Each lot of PromoCell Mononuclear Cell Medium is checked for unaltered shortterm maintenance of highly viable mononuclear cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium.

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C. Note: The SupplementMix is delivered thawed and can be frozen after arrival without losing any activity

Subculture Routine

Click here for more information.

Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary Human Mononuclear Cells and Growth Media

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

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Protocols

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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