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C-12907

Sigma-Aldrich

Human Mononuclear Cells (hMNC)

Isolated from peripheral blood, single donor, ultra-pure, 25 million cryopreserved cells

biological source

human peripheral blood

packaging

pkg of 25,000,000 cells

morphology

(mononuclear)

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Blood

Application

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

Quality

Rigid quality control tests are performed for each lot of Mononuclear Cells. They are routinely characterized by flow cytometry analyzing a series of cellular parameters, e.g. viability, cell size, granularity. provides detailed information on the percentages of the major cellular sub-populations, i.e., the lymphocytes, the monocytes, and granulocytes, for each lot of Mononuclear Cells. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 1 x 106 cells per mlTested Markers: CD31 negative, CD34 negative, CD105 positive, CD146 positive

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Articles

Primary Human Mononuclear Cells and Growth Media

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Protocols

Promocell

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

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