C-12620

Human Nasal Epithelial Cells (HNEpC)

500,000 cryopreserved cells

• Synonym(s): HNEpC cells

Properties

• biological source: human nose (nasal mucosa)
• packaging: pkg of 500,000 cells
• morphology: (epithelial)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Cell Line Origin

Nose

Application

Primary Human Nasal Epithelial Cells (HNEpC) are isolated from normal human nasal mucosa and are provided in a cryopreserved format. HNEpC stain positive for cytokeratin. The epithelium in the nasal cavity cleans, humidifies, and warms inhaled air. In addition, it produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. HNEpC are useful for in vitro studies of these processes.

Quality

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

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Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable