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C-12642

Sigma-Aldrich

Human Small Airway Epithelial Cells (HSAEpC)

500,000 cryopreserved cells

biological source

human (bronchiole)

packaging

pkg of 500,000 cells

morphology

(epithelial)

application(s)

cell culture | mammalian: suitable

relevant disease(s)

asthma

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Human Small Airway Epithelial Cells (HSAEpC) serve as model cell culture systems for in vitro immune response studies. The 3D culture of HSAEpC mimics the in vivo cells effectively. The 3D cells are more viable, functional and efficient for immune response studies compared to the 2D cells.
Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area and are provided in a cryopreserved format. They stain positive for cytokeratin.The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.

Cell Line Origin

Bronchiole

Application

Human Small Airway Epithelial Cells (HSAEpC) may be used:
  • in overexpression of αvβ6 integrin
  • in cytotoxicity studies with Cannabis sativa oil extract
  • in transfection studies with highly pathogenic avian influenza virus and influenza A virus

Quality

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: > 15

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Effects of cannabis oil extract on immune response gene expression in human small airway epithelial cells (HSAEpC): implications for chronic obstructive pulmonary disease (COPD)
Mamber SW, et al.
Journal of Cannabis Research, 2, 5-5 (2020)
Swantje Thiele et al.
Cell reports, 31(3), 107549-107549 (2020-04-23)
Importin-α adaptor proteins orchestrate dynamic nuclear transport processes involved in cellular homeostasis. Here, we show that importin-α3, one of the main NF-κB transporters, is the most abundantly expressed classical nuclear transport factor in the mammalian respiratory tract. Importin-α3 promoter activity...

Articles

Primary Small Airway Epithelial Cells and Growth Media

Primary human small airway epithelial cells and serum-free medium optimized for the in vitro cultivation of epithelial cells from the distal respiratory system. Protocols for handling, cell passaging, media, and product use.

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