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Key Documents

B2531

Sigma-Aldrich

Monoclonal Anti-BrdU antibody produced in mouse

clone BU-33, ascites fluid, Immunohistology Grade

Synonym(s):

Anti Brdu, Anti Brdu Antibody - Monoclonal Anti-BrdU antibody produced in mouse, Brdu Antibody, Anti-Bromodeoxyuridine

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BU-33, monoclonal

species reactivity

wide range

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using sections of intestine from mouse or rat treated in vivo with BrdU

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Monoclonal anti-BrdU (immunohistology grade) (mouse IgG1 isotype) is derived from the BU-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with bromodeoxyuridine conjugated to KLH.

Specificity

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Immunogen

bromodeoxyuridine conjugated to KLH.

Application

Monoclonal Anti-BrdU antibody has been used in immunostaining, BrdU incorporation into DNA, immunohistochemistry and uterine labeling index. It has also been used to investigate cell proliferation.

Biochem/physiol Actions

5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Storage and Stability

For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
B8434 Anti-BrdU antibody, Mouse monoclonal
clone BU-33, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alvaro Duque et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(42), 15205-15217 (2011-10-22)
As markers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous system. However, the effect of the incorporation of
David H and Huseyin M
Cell Proliferation and Apoptosis (2003)
Dietary Lutein/Zeaxanthin Decreases Ultraviolet B-Induced Epidermal Hyperproliferation and Acute Inflammation in Hairless Mice
Gonzalez S, et al.
The Journal of Investigative Dermatology, 121(2), 399-405 (2003)
The influence of dietary isoflavone on the uterotrophic response in juvenile rats
Michael GW, et al.
Food And Chemical Toxicology, 41(11), 1517-1525 (2003)
Short term culture of breast cancer tissues to study the activity of the anticancer drug taxol in an intact tumor environment
van der Kuip H, et al.
BMC Cancer, 6(1), 86-86 (2006)

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