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A8531

Sigma-Aldrich

Bovine Serum Albumin

pH <5.0, powder

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
MDL number:
eCl@ss:
42010201
NACRES:
NA.56

Quality Level

biological source

bovine

form

powder

mol wt

~66 kDa
~66,000

packaging

vial of 50 mg

technique(s)

ELISA: suitable
gel permeation chromatography (GPC): suitable
immunohistochemistry: suitable

impurities

~0.3% dithiothreitol

pH

<5.0

suitability

suitable for (Suitable for use as a gel filtration marker)

UniProt accession no.

storage temp.

−20°C

Gene Information

bovine ... ALB(280717)

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General description

Albumin is the most abundant plasma protein in humans. It has a molecular mass of 66.5 kDa.

Application

Gel filtration molecular weight marker
Albumin from bovine serum has been used:
  • for blocking of nonspecific immunoglobulin during immunohistochemical reactions.
  • to coat microplates for blocking non-specific binding during I-ELISA (Indirect Enzyme-linked Immunosorbent Assay)
  • as molecular mass standard in analytical gel filtration chromatography for Superdex® 75 10/300 GL gel filtration column.
  • for measuring fluorescence for PR1 (phytofluor red 1) by using Fluorescence correlation spectroscopy (FCS)
  • as a reference mixture along with horse Mb in Size Exclusion Chromatography
It can be used as a gel filtration molecular weight marker. It can be used in medical studies to quantify binding capacity and adsorption rate of protein enrichment and separation medium critical in proteomic research.

Biochem/physiol Actions

Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Albumin in serum level is associated with advanced liver disease.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Legal Information

Superdex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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