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A7511

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized

Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

assay

≥97% (agarose gel electrophoresis)

form

lyophilized powder

quality

crystallized

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

Fatty acid content

≤0.01%

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

pH

7

solubility

water: soluble (50 mg/ml)

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Packaging

1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Essentially fatty acid free
  • Crystallized

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. Further purified by a proprietary crystallization method
Prepared from albumin (A4378)
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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