A5963

Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

• Synonym(s): Anti-c-Myc, Monoclonal Anti-c-Myc−Alkaline Phosphatase antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: human
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: 1:100 using a myc-tagged fusion protein.
• isotype: IgG1
• UniProt accession no.: P01106
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MYC(4609)

Description

General description

Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62^c-myc is associated mainly with cell nuclei.

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Application

Anti-c-Myc-Alkaline Phosphatase antibody has been used in

• enzyme linked immunosorbent assay (ELISA)
• immunohistochemical labeling
• immunostaining
• western blotting

Monoclonal Anti-c-Myc-Alkaline Phosphatase antibody can be used for western blot assays at 1:100, using myc-tagged fusion proteins. The antibody can also be used for immunohistochemical applications.

Biochem/physiol Actions

The c-myc oncogene product(p62^c-myc) exerts normal and oncogenic cellular functions. Elevated level of c-myc protein in malignant tissues when compared with normal tissue, but with the unexpected finding of a cytoplasmic accumulation of the protein in these tumors.

Physical form

Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)