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A5378

Sigma-Aldrich

Albumin from chicken egg white

lyophilized powder, ≥90% (agarose gel electrophoresis)

Synonym(s):

Albumin from hen egg white, Ovalbumin

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About This Item

CAS Number:
9006-59-1
EC Number:
232-692-7
MDL number:
MFCD00130424
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

chicken egg white

Quality Level

200

type

Grade III

assay

≥90% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

chromatography

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 50 mg/mL

UniProt accession no.

P01012

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

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General description

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.

Application

The product is useful as a marker (~45 kDa) for protein electrophoresis. It has been used to produce an animal model of asthma. Ovalbumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil. It was also used in a study testing intestinal macromolecule absorption in the fetal pig after infusion of colostrum in utero.
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1A

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaodong Tong et al.
Biotechnology progress, 24(3), 714-719 (2008-04-24)
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we
Qian He et al.
Frontiers in immunology, 11, 571248-571248 (2020-10-20)
As the entry sites of many pathogens such as human immunodeficiency virus (HIV), mucosal sites are defended by rapidly reacting resident memory T cells (TRM). TRMs represent a special subpopulation of memory T cells that persist long term in non-lymphoid
A differential scanning calorimetric study of conversion of ovalbumin to S-ovalbumin in eggs.
J W Donovan et al.
Journal of the science of food and agriculture, 27(2), 197-204 (1976-02-01)
Christianne Bandeira-Melo et al.
Journal of immunological methods, 276(1-2), 227-237 (2003-05-10)
Eosinophils contain several preformed cytokines within their specific granules. Therefore, without requiring them in de novo synthesis of cytokines, eosinophils can release quantities of granule-derived cytokines by highly regulated mechanisms. However, eosinophil "degranulation" is poorly understood, in part, because available
David J Ferland et al.
Vascular pharmacology, 88, 30-41 (2016-11-29)
Chemerin is an adipokine associated with increased blood pressure, and may link obesity with hypertension. We tested the hypothesis that chemerin-induced contraction of the vasculature occurs via calcium flux in smooth muscle cells. Isometric contraction of rat aortic rings was
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