A2831

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Anti-Dinitrophenyl, Monoclonal Anti-DNP
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SPE-7, monoclonal
• form: buffered aqueous glycerol solution
• technique(s): direct ELISA: 1:25,000 using DNP-BSA; dot blot: 1:180,000 (chemiluminescence)
• isotype: IgE
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Immunogen

DNP-KLH conjugate

Application

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)