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T0956

Sigma-Aldrich

Sigma Universal Transfection Reagent

Polymer reagent for transfecting common mammalian cell lines

Quality Level

grade

for molecular biology

sterility

aseptically filled

form

liquid (buffered aqueous solution)

usage

sufficient for 110 transfections (3.5 cm dish)
sufficient for 40 transfections (10 cm dish)
sufficient for 65 transfections (6 cm dish)

concentration

2 mg/mL

storage temp.

−20°C

General description

Sigma Universal Transfection Reagent is a unique formulation of a biodegradable, proprietary cationic polymer conjugated to a cationic lipid. Universal Transfection Reagent is used for transfection of nucleic acids into common eukaryotic cell lines. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics. For most cells, medium change after transfection is not necessary.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 1-3 μl Universal Transfection Reagent per μg DNA. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Sigma Universal Transfection Reagent:

Excellent for:
BHK-21
CHO
CHOK1
HEK293
HeLa
NIH3T3

Good for:
3LL
6CSFME
ATT20B16-F0
B16-F10
BAEC
Ca Ski
COS-7
CV-1
D 407
HUVEC
L929
MCF-7
MDCK
Neuro2A
SAOS-2
SHEP
SiHa
SKOV3

Features and Benefits

  • Fast, easy protocol
  • Suitable for stable and transient transfection
  • High efficiency for a wide variety of cell types including primary cells
  • Compatible with both serum and serum-free transfection protocols

Components

Note the formulation of Sigma Universal Transfection Reagent is confidential.

Principle

Sigma Universal Transfection Reagent is a cationic polymer conjugated to a cationic lipid. When mixed with plasmid DNA, the reagent forms stable complexes that can be directly added to the cell culture. The cells take up the complexes by endocytosis, the polymer is cleaved from the complex, and the plasmid DNA is quickly released into the cytoplasm.

Other Notes

Complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum lowers the toxicity of transfection.

Certificate of Analysis

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Certificate of Origin

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