50 % (w/v) in H2O
gel permeation chromatography (GPC): suitable
single component solution
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10 - Combustible liquids
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
This product is nominally a 50% w/v solution of PEI in water, meaning 50 grams of poly(ethyleneimine) per 100 mL of solution. Concentrations of polymer solutions (such as PEI) are commonly reported either as a percentage or mg/mL, not in terms of molarity.
Mn is the number average molecular weight. This corresponds to the "true average" or mean; that is, the sum of the molecular weights divided by the number of molecules. Mw is the weight average molecular weight. This corresponds to the median value; that is, there are as many molecules smaller than the weight average molecular weight as there are larger than the weight average molecular weight. The fact that the two molecular weight values are so different means that there is a large, non-symmetrical distribution of molecular weights; the largest molecules probably have a molecular weight of several million.
This product is a polymer with a large molecular weight distribution. It is not amenable to being analyzed by typical chromatographic assays such as HPLC or TLC. Therefore, we do not report an overall purity value for this product.
For enzyme isolation, see:J.J. Jendrisak and R.R. Burgess, Biochem., 14(21), 4639-4645 (1975), "A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II".This paper describes preparing a 10% stock solution, which was adjusted to pH 7.9 by adding hydrochloric acid.PEI has also been used for the precipitation of nucleic acids in the purification process of aminoacyl-tRNA synthetases:von der Haar, F., "Purification of Aminoacyl-tRNA Synthetases", Methods in Enzymology, 59, 257-267 (1979).For transfections, see the following references:Boussif, O. et al., "A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine." Proc. Natl. Acad. Sci. USA, 92(16), 7297-7301 (1995).Thomas, M. and Klibanov, A.M., "Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells." Proc. Natl. Acad. Sci. USA, 100(16), 9138-9143 (2003).
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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