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Key Documents

S7899

Sigma-Aldrich

Sodium acetate buffer solution

pH 5.2±0.1 (25 °C), for molecular biology, 3 M, 0.2 μm filtered

Synonym(s):

Sodium acetate buffer 3M

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About This Item

CAS Number:
Beilstein/REAXYS Number:
3786729
MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

form

liquid

concentration

3 M

pH

5.2±0.1 (25 °C)

suitability

suitable for DNA precipitation

storage temp.

room temp

SMILES string

[Na+].CC(O)=O.CC([O-])=O

InChI

1S/2C2H4O2.Na/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+1/p-1

InChI key

BHZOKUMUHVTPBX-UHFFFAOYSA-M

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General description

Sigma′s Sodium Acetate Buffer is a 3M solution prepared with 18 megaohm water and filter-sterilized.

Application

Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay and in vitro enzyme inhibition assay.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual, A1-A1 (2001)
Saika Siddiqui et al.
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Tomas Erban et al.
Scientific reports, 7(1), 5084-5084 (2017-07-13)
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA
Sena Bluemel et al.
Gut microbes, 11(3), 265-275 (2019-04-16)
Alcohol-induced liver disease is closely related to translocation of bacterial products and bacteria from the intestine to the liver. However, it is not known whether bacterial translocation to the liver depends on certain intestinal microbiota changes that would predispose bacteria
Chun-Chun Chen et al.
Journal of visualized experiments : JoVE, (62)(62), doi:10-doi:10 (2012-05-09)
Knowing the timing, level, cellular localization, and cell type that a gene is expressed in contributes to our understanding of the function of the gene. Each of these features can be accomplished with in situ hybridization to mRNAs within cells.

Articles

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Protocols

Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography

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