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L4919

Sigma-Aldrich

Lysozyme from chicken egg white

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

grade

for molecular biology

Quality Level

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

lyophilized powder

specific activity

≥40,000 units/mg protein

mol wt

single-chain 14.3 kDa

composition

Protein, ≥90%

storage temp.

−20°C

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General description

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.[00]

Application

Lysozyme from chicken egg white has been used:
  • in dielectric spectroscopy studies of dynamics of protein
[01]
  • as a control to measure the human lysozyme activity
  • [02]
  • as a supplement in soaking solution to treat lenses
  • [04]
    Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

    Packaging

    500 mg in poly bottle
    1, 5 g in poly bottle

    Biochem/physiol Actions

    Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

    The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
    Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.[00]

    Features and Benefits

    • Highly purified by repeated crystallization and dialysis
    • Each lot is use-tested for isolation of plasmid DNA from E. coli

    Unit Definition

    One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

    Preparation Note

    3× crystallized

    Pictograms

    Health hazard

    Signal Word

    Danger

    Hazard Statements

    Precautionary Statements

    Hazard Classifications

    Resp. Sens. 1

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

    More Documents

    SDS

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