Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
8B - Non-combustible, corrosive hazardous materials
Enter Lot Number to search for Certificate of Analysis (COA).
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
One bottle of the product makes 1 liter of working solution. The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
Once diluted the solution should be stable indefinitely. It should be stored in the refrigerator and shaken well before each use. The solution tends to precipitate on standing and heat accelerates precipitation. Therefore, do not leave the bottle at room temperature overnight.
Use with nitrocellulose is not recommended, due to the methanol.
The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity.