MilliporeSigma
  • B2025
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B2025

Sigma-Aldrich

Brilliant Blue G - Colloidal Concentrate

NACRES:
NA.85

form

solution

Quality Level

storage temp.

room temp

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This Item
73142SCT027B3269
form

solution

form

powder

form

-

form

lyophilized powder

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

200

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion
  • ovarian tumor fluid proteome prior to profiling
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Reconstitution

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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