90A1

Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit

Select reagents packaged in gelatin capsules.

• MDL number: MFCD08705438
• UNSPSC Code: 41116123
• NACRES: NA.47

Properties

• Quality Level: 500
• form: liquid
• shelf life: Expiry date on the label.
• IVD: for in vitro diagnostic use
• application(s): hematology; histology
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

Kit Components Only

• α-Naphthyl acetate (90-6) 10 x 20
• Citrate solution (386-1) 20 mL
• Ethylene glycol monomethyl ether 25 mL
• Fast Blue RR Salt (FBS-25) 10 caps
• Mayer's Hematoxylin Solution (MHS-1) 100 mL
• TRIZMAL™ 7.6 Concentrate 50 mL

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H302 + H312 + H332,H314,H360FD,H370,H373
• pcodes: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - STOT RE 2 - STOT SE 1
• target_organs: Immune system, thymus
• Storage Class: 3 - Flammable liquids
• flash_point_f: 104.0 °F - closed cup
• flash_point_c: 40 °C - closed cup