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89149

Sigma-Aldrich

Fluorescent IEF-Marker pI 5.2 solution

1 mg/mL in 5 mM HCl

Synonym(s):

IEF-Marker fluorescent pI 5.2

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About This Item

UNSPSC Code:
41105336
NACRES:
NA.26

form

liquid

mol wt

Mw 285.30 g/mol

concentration

1 mg/mL in 5 mM HCl

fluorescence

λex 336 nm; λem 424 nm (in 0.1 M citrate pH 5.2)

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

storage temp.

2-8°C

General description

filtered through a 0.45 μm membrane filter

Application

suitable as IEF-Marker for HPCE and for IEF-gels

Reconstitution

For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1 μl per lane).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


Certificates of Analysis (COA)

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Articles

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

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