MilliporeSigma
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71768

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

suitable for enzyme immunoassay, ≥99.0% (enzymatic)

Synonym(s):
p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
Beilstein:
5324661
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

assay

≥99.0% (enzymatic)

form

powder or crystals

technique(s)

enzyme immunoassay: suitable

impurities

≤0.1% free nitrophenol

storage temp.

2-8°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity
  • as substrate to assay acid phosphatase activity
  • as substrate to assay bound antibodies with anti-rabbit/human alkaline phosphatase conjugate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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