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67060

Sigma-Aldrich

Methyl Green

zinc chloride salt, for microscopy (Bact., Bot., Hist.)

Synonym(s):
Ethyl Green
Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
Molecular Weight:
653.24
Colour Index Number:
42590
Beilstein:
3914106
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Bact., Bot., Hist.)

Quality Level

form

powder

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥900 at 624-634 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

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Application

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Biochem/physiol Actions

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Analysis Note

Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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