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215597

Sigma-Aldrich

Basic Fuchsin

Dye content >85 %

Synonym(s):

Basic Parafuchsin, Basic Red 9, Magenta O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C19H17N3 · HCl
CAS Number:
Molecular Weight:
323.82
Colour Index Number:
42500
Beilstein/REAXYS Number:
4164603
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

assay

>85.0% (HPLC)

form

solid

composition

Dye content, >85%

mp

268-270 °C (dec.) (lit.)

λmax

545 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

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General description

Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

Application

Basic fuchsin is utilized for various applications:
  • Acid-fast staining
  • Periodic Acid-Schiff
  • Feulgen stain for DNA
  • ENDO culture medium
  • Leifson′s method for bacterial flagella

Biochem/physiol Actions

Basic fuchsin displays anesthetic, bactericidal (gram-positive), and fungicidal properties.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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Journal of Biomedical Materials Research Part A, 68, 123-132 (2004)
Bisen PS
Laboratory Protocols in Applied Life Sciences (2014)
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Plant Fibers, 10 (2012)
I A Schrijver et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(1), 95-103 (2000-02-01)
Integral immunohistochemical analysis of immune responses in frozen sections requires that, in addition to constitutively expressed membrane CD markers, less stable determinants can be reliably visualized. Therefore, we compared the commonly used acetone fixation method with pararosaniline fixation for six

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