All Photos(2)

857343

Sigma-Aldrich

Basic Fuchsin

certified by the Biological Stain Commission, Dye content ≥88 %

Synonym(s):
Parafuchsin hydrochloride, Pararosaniline hydrochloride, Magenta O, Paramagenta hydrochloride, Basic Red 9, Pararosaniline chloride, Basic Parafuchsin
Empirical Formula (Hill Notation):
C19H17N3 · HCl
CAS Number:
Molecular Weight:
323.82
Colour Index Number:
42500
Beilstein:
4164603
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥88%

pH range

1.0 - 3.1, purple to red

mp

268-270 °C (dec.) (lit.)

λmax

544 nm

ε (extinction coefficient)

≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L
≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Suitability

Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Legal Information

Magenta is a trademark of Magenta Corp.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Min Chen et al.
The New phytologist, 229(6), 3481-3496 (2020-11-25)
The intimate association of host and fungus in arbuscular mycorrhizal (AM) symbiosis can potentially trigger induction of host defence mechanisms against the fungus, implying that successful symbiosis requires suppression of defence. We addressed this phenomenon by using AM-defective vapyrin (vpy)
Plant Fibers (2012)
Bisen PS
Laboratory Protocols in Applied Life Sciences (2014)
Sharma A
Plant Chromosomes (1999)
G Vinitha et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 68(1), 1-5 (2006-12-13)
Solid-state dye-doped polymers are attractive alternative to the conventional liquid dye solutions. In this paper, nonlinear properties of the dye Pararosanilin has been studied. The third-order nonlinear optical properties of Pararosanilin dye in 1-butanol and dye-doped polymer film were measured

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service