42364

1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate

BioReagent, suitable for fluorescence, ≥98.0% (TLC)

• Synonym(s): DiI
• Empirical Formula (Hill Notation): C₅₉H₉₇ClN₂O₄
• CAS Number: 41085-99-8
• Molecular Weight: 933.87
• Beilstein: 8251870
• MDL number: MFCD00142354
• PubChem Substance ID: 57650110
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• assay: ≥98.0% (TLC)
• mp: 68 °C (dec.) (lit.)
• solubility: DMF: soluble; DMSO: soluble; methanol: soluble
• fluorescence: λ_ex 550 nm; λ_em 567 nm in phosphate buffer/SDS pH 7.0
• suitability: suitable for fluorescence
• SMILES string: [O-]Cl(=O)(=O)=O.CCCCCCCCCCCCCCCCCCN1c2ccccc2C(C)(C)/C1=C\C=C\C3=[N+](CCCCCCCCCCCCCCCCCC)c4ccccc4C3(C)C
• InChI: 1S/C59H97N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-35-41-50-60-54-46-39-37-44-52(54)58(3,4)56(60)48-43-49-57-59(5,6)53-45-38-40-47-55(53)61(57)51-42-36-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h37-40,43-49H,7-36,41-42,50-51H2,1-6H3;(H,2,3,4,5)/q+1;/p-1
• InChI key: JVXZRNYCRFIEGV-UHFFFAOYSA-M

Description

General description

DiI is a Dye Aye, a chemical with CAS number 41085-99-8. It is a fluorescent lipophilic cationic indocarbocyanine dye, which is usually made as a perchlorate salt. Mostly used for scientific staining purposes, such as single molecule imaging, fate mapping and neuronal tracing (as DiI is retained in the lipid bilayers).

Application

1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate is used for the following applications:

• Preparation of Dil solution
• fluorescent membrane dye
• Di-I Staining

Packaging

100, 500 mg

Biochem/physiol Actions

Lipophilic carbocyanine dye used primarily for optical recordings of membrane voltage and for studies of membrane fluidity. Retrograde stain for neurons; provides intense, long-lasting staining of live neurons in vivo and in vitro.

Other Notes

Lipophilic fluorescent probe for studying membranes

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves