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12306C

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonym(s):
FCS, serum, FBS, sera
MDL number:
NACRES:
NA.71

Quality Level

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Premier

quality

from USDA approved countries

composition

Hemoglobin, ≤25 mg/dL

origin

non-USA origin

application(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.
FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Suitability

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
Asgari S, et al.
Stem Cell Reviews and Reports, 9(4), 493-504 (2013)
Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
Vosough M, et al.
Stem Cells and Development, 22(20), 2693-2705 (2013)
Gerhard Gstraunthaler
ALTEX, 20(4), 275-281 (2003-12-13)
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The...
Xiao-Min Liu et al.
Nature chemical biology, 15(9), 865-871 (2019-08-07)
RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A...
Yusuke Ohnishi et al.
PloS one, 3(5), e2248-e2248 (2008-05-22)
Allele-specific gene silencing by RNA interference (RNAi) is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi), the design and assessment of small interfering RNA...

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