All Photos(1)
biological source
fish brain
Quality Level
growth mode
Adherent
karyotype
Not specified
morphology
Squamous
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Common carp brain cell line, koi herpesvirus isolation
Cell Line Description
The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.
Culture Medium
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). 10 mM HEPES can be included if a CO2 incubator is not available.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:6, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
Enter Lot Number to search for Certificate of Origin (COO).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service