MilliporeSigma
All Photos(2)

1321N1 Cell Line human

86030402, from human brain(astrocytoma)

Synonym(s):
1321-N1 Cells

biological source

human brain (astrocytoma)

growth mode

Adherent

karyotype

Not specified

morphology

Glial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human brain astrocytoma

Cell Line Description

1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten .1321N1 has been shown to have very similar STR profile data to U-118 MG.

Application

Human 1321N1 Cell Line has been used to study how the multiscale morphology of functional thin films affects the in vitro behavior of human neural astrocytoma 1321N1 cells. It has also been used to study the expression and reactivity of Caytaxin protein.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service