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Dimethyl sulfoxide

ReagentPlus®, ≥99.5%

Linear Formula:
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line






autoignition temp.

573 °F

expl. lim.

42 %, 63 °F


immunoblotting: suitable


≤0.1% water (Karl Fischer)

refractive index

n20/D 1.479 (lit.)


189 °C (lit.)


16-19 °C (lit.)


H2O: soluble(lit.)
acetone: soluble(lit.)
benzene: soluble(lit.)
chloroform: soluble(lit.)
ethanol: soluble(lit.)


1.10 g/mL (lit.)

SMILES string




InChI key


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General description

Dimethyl sulfoxide is an amphipathic molecule, soluble in both aqueous and organic solvents. It is commonly used as an aprotic solvent and also as a reagent in organic synthesis.


DMSO has been used as a medium to prepare a chemiluminescence reagent that can be a substitute to commercially-available enhanced chemiluminescence kits for use in immunoblotting technique.


100, 500 mL in glass bottle
1, 6×1, 2.5, 4 L in glass bottle
5, 10 L in poly drum
Note: D5879-1L in glass bottle /D5879-1L-D in poly bottle.

Biochem/physiol Actions

DMSO is a highly polar substance that can be used as an industrial solvent for organic and inorganic chemicals. It is also used as a protectant during cold storage of living cells. It is also useful for the oxidation of thiols and disulfides to sulfonic acids.


Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids



Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molarity of Product D5879, Dimethyl sulfoxide?

    Pure solvents do not technically have a molarity (defined as moles of solute per liter of solution), since there is no solute distinct from the pure material. However, an effective molar concentration of 14.1M can be determined for this product, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  6. The Dimethyl sulfoxide, Product D5879,  I ordered arrived as a solid. Is the product still acceptable?

    Sigma-Aldrich ships many products, including DMSO, at ambient temperature. As a result product D5879 might arrive as a solid depending on the weather conditions during shipment. The melting point of DMSO is 16-19°C. Therefore, if the solid is allowed to warm to room temperature, it will melt without any impact on the quality of the product.

  7. My application requires a dry solvent. How can I remove water that is present in Dimethyl sulfoxide, Product D5879?

    This product has a water content specification of not more than 0.1%, which may be low enough for your application. Other DMSO products may have more water present. Molecular sieves are typically zeolite compounds that strongly adsorb water and have carefully controlled pore sizes. If you would like, dried sieves may be placed into the bulk solvent container (about 5%-10% by volume) to remove most water.  Sigma-Aldrich offers 4 Å molecular sieves (e.g. product 334308), which would be effective in removing residual water from DMSO.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Levent Keskintepe et al.
Journal of assisted reproduction and genetics, 26(11-12), 629-635 (2009-12-08)
Cryopreservation of blastocysts, especially those subjected to the trauma due to blastomere biopsy for the purposes of pre-implantation genetic screening (PGS), requires significant optimization. Laboratory and clinical outcomes were compared to determine the effect of two different cryopreservation techniques on
Julia L Zehr et al.
Biology of reproduction, 72(5), 1087-1094 (2004-12-31)
This study investigated whether prenatal androgen exposure, social rank, and body weight are factors regulating pubertal development in outdoor-housed female rhesus monkeys. Subjects' mothers received injections of testosterone enanthate (20 mg/ wk), flutamide (an androgen receptor blocker, 30 mg/kg twice
M Bouquet et al.
Molecular reproduction and development, 40(1), 110-115 (1995-01-01)
In a previous study, we have shown that the cryopreservation of mouse oocytes caused increases in the rates of degeneration and of digynic polyploid embryos, while the fertility of frozen-thawed oocytes was decreased. In this study, we have attempted to
Sun-A Ock et al.
Cell transplantation, 20(8), 1231-1239 (2011-02-08)
Dimethyl sulfoxide (DMSO), a commonly used cryoprotectant in cryopreservation procedures, is detrimental to viability of cells. In this view point, a comparative study was carried out to evaluate the effect of DMSO on porcine mesenchymal stem cells (pMSCs). We compared
M Ruhnke et al.
Journal of clinical microbiology, 32(9), 2092-2098 (1994-09-01)
After repeated use of fluconazole for therapy of oropharyngeal candidosis, the emergence of in vitro fluconazole-resistant Candida albicans isolates (MIC, > or = 25 micrograms/ml) together with oral candidosis unresponsive to oral dosages of up to 400 mg of fluconazole

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