650498

Sigma-Aldrich

Chloroform

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein/REAXYS Number:
1731042
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.04

Quality Level

grade

HPLC Plus
for residue analysis

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.9%

form

liquid

contains

amylenes as stabilizer

application(s)

HPLC: suitable
gas chromatography (GC): suitable

impurities

≤0.02% water
≤0.5 ppb Fluorescence (quinine) at 365 nm

evapn. residue

≤0.0001%

halogenated residue

<10 ng/L (as heptachlor epoxide)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

λ

H2O reference

UV absorption

λ: 245 nm Amax: 1.00
λ: 250 nm Amax: 0.30
λ: 275-400 nm Amax: 0.005

Featured Industry

Food and Beverages

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Application

Suitable for HPLC, spectrophotometry, environmental testing
Chloroform was used in the making of silanized coverslips for DNA combing.
It may be used as:
  • Extractant in solvent extraction process.
  • A component of the binary mobile phases for liquid chromatography–atmospheric pressure photoionization-mass spectrometry (LC–APPIMS) of triacylglycerol model compounds.
  • Solvent for recrystallization.

Packaging

1, 6×1, 4, 4×4 L in glass bottle

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Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

hazcat

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

storage_class_code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK Germany

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Silanization of coverslips for DNA combing.
Labit H, et al.
Biotechniques, 11, 50-50 (2009)
Sheng-Suan Cai et al.
Journal of chromatography. A, 1173(1-2), 88-97 (2007-10-30)
In this work, we evaluate the performance of liquid chromatography-atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS) for non-aqueous reversed phase analysis of six triacylglycerol model compounds using six binary mobile phases including MeOH/iPrOH, MeOH/CHCl(3), MeOH/CH(2)Cl(2), CH(3)CN/iPrOH, CH(3)CN/CHCl(3), and CH(3)CN/CH(2)Cl(2). All mobile phases...
Electrogenerated chemiluminescent emission from an organized (L-B) monolayer of a Ru(bpy)32+-based surfactant on semiconductor and metal electrodes.
Zhang X and Bard AJ.
The Journal of Physical Chemistry, 92(20), 5566-5569 (1988)
Analytical chemistry in a drop. Solvent extraction in a microdrop.
Liu H and Dasgupta PK.
Analytical Chemistry, 68(11), 1817-1821 (1996)
Kuan Sun et al.
Nature communications, 6, 6013-6013 (2015-01-15)
Solution-processed organic photovoltaic cells (OPVs) hold great promise to enable roll-to-roll printing of environmentally friendly, mechanically flexible and cost-effective photovoltaic devices. Nevertheless, many high-performing systems show best power conversion efficiencies (PCEs) with a thin active layer (thickness is ~100 nm) that...

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