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Ascorbic Acid

Pharmaceutical Secondary Standard; Certified Reference Material

L-Ascorbic acid, Antiscorbutic factor, Vitamin C, L-Threoascorbic acid
Empirical Formula (Hill Notation):
CAS Number:
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EC Number:
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PubChem Substance ID:
E Number:

Quality Level


certified reference material
pharmaceutical secondary standard


traceable to BP 461
traceable to Ph. Eur. A1300000
traceable to USP 1043003




current certificate can be downloaded


HPLC: suitable
gas chromatography (GC): suitable


190-194 °C (dec.)


food and beverages



storage temp.


SMILES string




InChI key


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General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. Ascorbic acid is a powerful antioxidant and is widely used in pharmaceutical formulations. It has proven to have a beneficial effect on age-related diseases such as cancer, atherosclerosis, ocular, neurodegenerative diseases, etc. It is mainly involved in the formation and maintenance of collagen. The prolonged deficiency of ascorbic acid in diet may result in scurvy.


Ascorbic Acid may be used as a pharmaceutical reference standard for the quantification of the analyte in pharmaceutical formulations using hydrophilic interaction chromatography.

Biochem/physiol Actions

Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAC2886 in the slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

Method development and validation for optimised separation of salicylic, acetyl salicylic and ascorbic acid in pharmaceutical formulations by hydrophilic interaction chromatography and response surface methodology
Hatambeygi N, et al.
Journal of Chromatography A, 1218(35), 5995-6003 (2011)
Simultaneous differential pulse voltammetric determination of ascorbic acid and caffeine in pharmaceutical formulations using a boron?doped diamond electrode
Lourencao CB, et al.
Electroanalysis, 22(15), 1717-1723 (2010)
L-ascorbic acid determination in pharmaceutical formulations using a biosensor based on carbon paste modified with crude extract of Zucchini (Cucurbita pepo)
Fatibello-Filho and Vieira CI
Journal of the Brazilian Chemical Society, 11(4), 412-418 (2000)
Marc P Bradshaw et al.
Critical reviews in food science and nutrition, 51(6), 479-498 (2011-09-21)
Extensive reviews of research are available on the use of ascorbic acid, and its consequent degradation pathways, in physiological conditions or food matrices. However, very little information can be found for wine-related systems. This review highlights the relevant chemistry and
Kathryn Blaschke et al.
Nature, 500(7461), 222-226 (2013-07-03)
DNA methylation is a heritable epigenetic modification involved in gene silencing, imprinting, and the suppression of retrotransposons. Global DNA demethylation occurs in the early embryo and the germ line, and may be mediated by Tet (ten eleven translocation) enzymes, which convert 5-methylcytosine


HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide

Separation of (L)-Dehydroascorbic acid; L-Ascorbic acid 20 μg/mL

HPLC Analysis of Vitamins, Water Soluble on Discovery® C18

Separation of Pyridoxine; Nicotinamide; Nicotinic acid; Folic acid; (−)-Riboflavin, meets USP testing specifications; L-Ascorbic acid

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