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89640

Sigma-Aldrich

Toluidine Blue

for microscopy (Hist., Vit.)

Empirical Formula (Hill Notation):
C15H16ClN3S · 0.5ZnCl2
CAS Number:
Molecular Weight:
373.97
Colour Index Number:
52040
Beilstein:
4068052
MDL number:
PubChem Substance ID:
NACRES:
MA.02

Quality Level

grade

for microscopy (Hist., Vit.)

form

powder or crystals

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

εmax

≥800 at 621-636 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C

InChI

1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2

InChI key

DAQXTSXITPUGRG-UHFFFAOYSA-L

General description

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals. It is found to have affinity for nucleic acids, and therefore binds to nuclear material of tissues with a high DNA and RNA content.

Application

Toluidine Blue has been used as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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More Documents

Quotes and Ordering

Stephen H Schlecht et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(9), 1910-1919 (2019-05-02)
Postnatal development and the physiological loading response of the anterior cruciate ligament (ACL) complex (ACL proper, entheses, and bony morphology) is not well understood. We tested whether the ACL-complex of two inbred mouse strains that collectively encompass the musculoskeletal variation
Xiaoli Guo et al.
PLoS pathogens, 13(2), e1006142-e1006142 (2017-02-06)
Stem cell pools in the SAM (shoot apical meristem), RAM (root apical meristem) and vascular procambium/cambium are regulated by CLE-receptor kinase-WOX signaling modules. Previous data showed that cyst nematode CLE-like effector proteins delivered into host cells through a stylet, act
Yu Han et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Rosa chinensis is one of the most popular flower plants worldwide. The recurrent flowering trait greatly enhances the ornamental value of roses, and is the result of the constant formation of new flower buds. Flower bud differentiation has always been
A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone.
Zhu H, et al.
Nature Protocols, 5, 550-560 (2010)
Toluidine blue: A review of its chemistry and clinical utility.
Sridharan G and Shankar AA
International Journal of Oral and Maxillofacial Pathology, 16(2), 251-255 (2012)

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