03777

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

BioUltra, for molecular biology, ≥99.0% (T)

• Synonym(s): EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
• Linear Formula: [-CH₂OCH₂CH₂N(CH₂CO₂H)₂]₂
• CAS Number: 67-42-5
• Molecular Weight: 380.35
• Beilstein: 1717370
• EC Number: 200-651-2
• MDL number: MFCD00004291
• PubChem Substance ID: 329747807
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 300
• product line: BioUltra
• Assay: ≥99.0% (T)
• form: powder
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.05% (as SO₄)
• loss: ≤1.0% loss on drying, 110 °C
• mp: 241 °C (dec.) (lit.)
• solubility: 0.1 M NaOH: 0.1 M at 20 °C, clear, colorless
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤200 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• absorption: cut-off at 263 nm in 1 M NaOH at 0.1 M
• SMILES string: OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
• InChI key: DEFVIWRASFVYLL-UHFFFAOYSA-N

Description

Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid can be used to study molecular biology, biochemicals, reagents and chelators. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used in a study that illustrated the response of the β-cyclodextrin-hemin complex in the induction of cucumber adventitious rooting through heme oxygenase-1-dependent mechanism and calcium signaling.

A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Other Notes

Buffer component in dideoxy sequencing of RNA using reverse transcriptase.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves