SFABIKB
Roche
KAPA SYBR® FAST
suitable for qPCR, 2 ×, ABI PRISM®
Synonym(s):
qPCR
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
Quality Level
usage
sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions
shelf life
≤12 mo.
feature
dNTPs included
hotstart
packaging
kit of 1 mL (100 x 20 μL rxn; KK4603)
kit of 10 mL (1000 x 20 μL rxn; KK4605)
kit of 5 mL (500 x 20 μL rxn; KK4604)
kit of 50 mL (5000 x 20 μL rxn; KK4617)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
qPCR: suitable
input
purified DNA
compatibility
for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with StepOne™
for use with StepOnePlus™
detection method
SYBR® Green
storage temp.
−20°C
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General description
KAPA SYBR® FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
Application
KAPA SYBR® FAST qPCR kit can be used for:
- Gene expression
- Gene knockdown validation
- Microarray validation
- ChIP analysis
- Low copy detection
- Absolute quantification of NGS libraries
- Real-time PCR
Features and Benefits
Quantitate changes in gene expression more accurately
Detect low copy and difficult targets consistently
Complete real-time PCR runs in just 40 minutes
Quick Notes:
- High reaction efficiency between 95 – 105% improves accuracy and reproducibility.
- Unbiased efficiency across a wide range of GC contents and amplicon lengths.
Detect low copy and difficult targets consistently
- Improved processivity results in earlier Cq scores.
- Higher fluorescence detection across varying AT- and GC-rich targets.
- Novel enzyme is resistant to SYBR® inhibition.
Complete real-time PCR runs in just 40 minutes
- 55% shorter run times with fast cycling protocol.
- Maintain high performance when switching from standard to fast protocols.
Quick Notes:
- This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
- The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
- 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
- Do not exceed 25 μL reaction volumes.
Quality
All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.
Preparation Note
Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Kit Components Only
Product No.
Description
- KAPA SYBR® FAST DNA Polymerase
- reaction buffer
- dNTPs
- SYBR® Green I dye
- MgCl2 2.5 mM
signalword
Warning
hcodes
Hazard Classifications
STOT SE 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
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