11427857910

Fluorescein-12-UTP

≥85% (HPLC), solution

• Synonym(s): Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
• Empirical Formula (Hill Notation): C₃₉H₄₁N₄O₂₂P₃
• CAS Number: 134367-01-4
• Molecular Weight: 1010.68
• UNSPSC Code: 41116100

Properties

• Quality Level: 100
• assay: ≥85% (HPLC)
• form: solution
• mol wt: 1034.4
• packaging: pkg of 25 μL (250 nmol; 10 mM)
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

General description

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).

Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C₃₉H₃₇N₄O₂₂P₃Li₄.

Application

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

Other Notes

For life science research only. Not for use in diagnostic procedures.

For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash