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SCC208

Sigma-Aldrich

INS-1 832/3 Rat Insulinoma Cell Line

INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.

Synonym(s):
INS1 832/3, INS-1 (832/3), 832/3
eCl@ss:
32011203

biological source

rat

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

General description

Glucose-stimulated insulin secretion (GSIS) is potentiated by pancreatic beta cells and is critical to the physiological control of blood glucose levels. Insulin secretion is impaired in type 2 diabetes, and insight into the mechanisms and regulation of insulin secretion is fundamental to understanding the roles of beta cells in metabolic disease.
The rat insulinoma cell line INS-1 is a well-established model for studies of pancreatic islet beta-cell function ; however, the GSIS response of INS-1 cells may decrease over time. The INS-1 832/3 cell line is a subclone of INS-1 that was selected for robust GSIS, producing and secreting both rat and human insulin. INS-1 832/3 harbors a human insulin expression cassette allowing for human insulin secretion to be maintained over extended passages with selection . INS-1 832/3 cells are widely used to study the mechanisms of cellular insulin secretion, storage and synthesis. INS-1 832/3 cells may be characterized by granular staining for synaptotagmin, as described for the parental cell line . The INS-1 832/3 cell line exhibits the unique feature of additional insulin secretion in response to natural incretin hormones such as glucagon-like peptide 1 (GLP1), pituitary adenylate cyclase-activating peptide (PACAP), and gastric inhibitory peptide (PIP) , making INS-1 832/3 a valuable tool for physiologically relevant investigations of insulin regulation.

Source:
INS-1 832/3 is a derivative of INS-1 cells originally established from an x-ray induced insulinoma in rat1. The INS-1 832/3 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human insulin expression plasmid carrying a geneticin (G418)-resistance marker for selection .

Cell Line Description

Cancer Cells

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
Research Sub Category
Diabetes
Research Category
Metabolism

Quality

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificate of Quality

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