MABT889
Anti-CTHRC1 Antibody, clone Vli-55
clone Vli-55, from rabbit
Synonym(s):
Collagen triple helix repeat-containing protein 1, Protein NMTC1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
Vli-55, monoclonal
species reactivity
rat, mouse, human, porcine
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Cthrc1(68588)
General description
Collagen triple helix repeat-containing protein 1 (UniProt Q96CG8; also known as Protein NMTC1) is encoded by the CTHRC1 gene (ORF UNQ762/PRO1550; Gene ID 115908) in human. CTHRC1 is a secreted glycoprotein with 12 repeats of the Gly-X-Y motif (a.a. 58-93 based on the pro-form seqeunce) that is reported to increase cell motility and promote cell migration by limiting the deposition of collagen matrix. CTHRC1 is normally not expressed in blood vessels, but is induced in adventitial cells in remodeling arteries, as well as in dermal fibroblasts during wound healing. Upregulatetd CTHRC1 level is also observed in many human solid tumors, including non-small-cell lung cancer (NSCLC), gastric cancer, hepatocellular cancer, breast cancer, pancreatic cancer, and colorectal cancer (CRC). CTHRC1 is initially produced with a signal peptide sequence (a.a. 1-30), the removal of which yields the 213-amino acid (a.a. 31-243) mature protein.
Specificity
Clone Vli-55 stained the midbrain region of wild-type, but not Cthrc1-null mice (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142). Expected to react with all three spliced isoforms of human CTHRC1 reported by UniProt (Q96CG8).
Immunogen
Epitope: Near C-terminus.
Linear peptide corresponding to a C-terminal sequence of human CTHRC1.
Application
Anti-CTHRC1 Antibody, clone Vli-55 is an antibody against CTHRC1 for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: Clone Vli-55 hybridoma culture supernatant (1:250 dilution) immunostained the paraventricular nucleus of the hypothalamus in mouse brain tissue sections (Courtesy of Dr. Volkhard Lindner, Tufts University School of Medicine, ME, USA).
Western Blotting Analysis: Representative lots detected exogenously expressed human and rat CTHRC1 in lysates from transfected cells (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449; Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Western Blotting Analysis: A representative lot detected the overexpressed Cthrc1 in plasma samples from Cthrc1 trangenic mice, as well as immunoprecipitated CTHRC1 from normal human plasma samples (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot detected increased Cthrc1 expression in interstitial cells and activated fibroblasts of various remodeling tissues from wild-type, but not Cthrc1-null mice following angiotensin II infusion by immunohistochemistry staining of paraformaldehyde-fixed, paraffin-embedded sections (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449).
Immunohistochemistry Analysis: Representative lots immunostained Cthrc1-positive cells in paraformaldehyde-fixed, paraffin-embedded human, pig, and rat pituitary gland tissue sections (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449; Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot immunostained adventitial cells of remodeling renal artery, dermal cells in skin wounds, embryonic cartilage, as well as midbrain of wild-type, but not Cthrc1-null mice, using paraformaldehyde-fixed, paraffin-embedded tissue sections (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot localized Cthrc1 immunoreactivity in various regions of paraformaldehyde-fixed, paraffin-embedded mouse and pig brain sections (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Western Blotting Analysis: Representative lots detected exogenously expressed human and rat CTHRC1 in lysates from transfected cells (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449; Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Western Blotting Analysis: A representative lot detected the overexpressed Cthrc1 in plasma samples from Cthrc1 trangenic mice, as well as immunoprecipitated CTHRC1 from normal human plasma samples (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot detected increased Cthrc1 expression in interstitial cells and activated fibroblasts of various remodeling tissues from wild-type, but not Cthrc1-null mice following angiotensin II infusion by immunohistochemistry staining of paraformaldehyde-fixed, paraffin-embedded sections (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449).
Immunohistochemistry Analysis: Representative lots immunostained Cthrc1-positive cells in paraformaldehyde-fixed, paraffin-embedded human, pig, and rat pituitary gland tissue sections (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449; Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot immunostained adventitial cells of remodeling renal artery, dermal cells in skin wounds, embryonic cartilage, as well as midbrain of wild-type, but not Cthrc1-null mice, using paraformaldehyde-fixed, paraffin-embedded tissue sections (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot localized Cthrc1 immunoreactivity in various regions of paraformaldehyde-fixed, paraffin-embedded mouse and pig brain sections (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Quality
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 10 µg/mL of this antibody detected CTHRC1 in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 10 µg/mL of this antibody detected CTHRC1 in 10 µg of human brain tissue lysate.
Target description
~25 kDa observed. 23.08 kDa (isoform 1), 25.16 kDa (isoform 2), 24.77 kDa (isoform 3) calculated.
Physical form
Format: Purified
Protein G Purified
Purified rabbit monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Huixin Li et al.
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Cancer-associated fibroblasts (CAFs) are continuously activated and are one of the most important cellular components of the tumor matrix. The role of CAFs in the tumor microenvironment has been widely recognized. However, the underlying molecular mechanism by which CAFs promote
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