MABS1923

Anti-PTK7 Antibody, clone 31G9

clone 31G9, from rat

• Synonym(s): Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 31G9, monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_002812
• UniProt accession no.: Q13308
• target post-translational modification: unmodified
• Gene Information: human ... PTK7(5754)

Description

General description

Inactive tyrosine-protein kinase 7 (UniProt: Q13308; also known as Colon carcinoma kinase 4, CCK-4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7, PTK7) is encoded by the PTK7 (also known as CCK4) gene (Gene ID: 5754) in human. PTK7 is a single-pass type I membrane protein that lacks the catalytic activity. It is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. It is weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Its expression is reported to be higher in cell lines established from normal non-tumorigenic tissues compared those from highly metastatic invasive carcinomas. Six isoforms of PTK7 have been described that are generated by alternative splicing. It is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate mature form. It contains seven Ig-like-C2 type domains. It has an extracellular domain (aa31-704), a transmembrane domain (aa 705-725; and a cytoplasmic domain (aa 726-1070). PTK7 is shown to associate with receptor tyrosine kinase-like orphan receptor 2 (ROR2) to form a heterodimeric complex in mammalian cells and PTK7 and ROR2 physically and functionally interact with the non-canonical WNT5A ligand leading to JNK activation and cell movements. PTK7 is cleaved by MMP14 between Pro 621 and Leu 622 to generate an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. (Ref.: Martinez, S., et al. (2015). J. Biol. Chem. 290(51); 30562-72).

Specificity

Clone 31G9 is a rat monoclonal antibody that detects human Inactive tyrosine protein kinase 7 (PTK7). It targets an epitope within the extracellular domain.

Immunogen

Epitope: extracellular domain

GST-tagged recombinant fragment corresponding to the extracellullar domain of human Inactive tyrosine-protein kinase 7 (PTK7).

Application

Anti-PTK7, clone 31G9, Cat. No. MABS1923, is a rat monoclonal antibody that detects Inactive tyrosine-protein kinase 7 (PTK7) and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunofluorescence Analysis: A 1:25 dilution from a representative lot detected PTK7 in human skin and human placenta tissues.

Immunofluorescence Analysis: A representative lot detected PTK7 in Immunofluorescence applications (Martinez, S., et. al. (2015). J Biol Chem. 290(51):30562-72).

Immunoprecipitation Analysis: A representative lot immunoprecipitated PTK7 in HEK293 cell lysate. (Martinez, S., et. al. (2015). J Biol Chem. 290(51):30562-72).

Western Blotting Analysis: A representative lot detected PTK7 in Western Blotting applications (Martinez, S., et. al. (2015). J Biol Chem. 290(51):30562-72).

Research Category
Signaling

Quality

Evaluated by Flow Cytometry in NCI-H460 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected PTK7 in one million NCI-H460 cells.

Target description

118.40 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1